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Arraystar inc rat circrna array
Rat Circrna Array, supplied by Arraystar inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rat+circrna+array/rat+circrna+array/pm38622592-67-1-9
Average 90 stars, based on 1 article reviews

rat circrna array - by Bioz Stars, 2026-11

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Article Title: Therapeutic Target MicroRNA Identification Based on Circular RNA Expression Signature After Intracerebral Hemorrhage.
Article Snippet: Intracerebral hemorrhage (ICH) is a devastating stroke subtype with grave neurological outcome [1].. A specific therapeutic modality for ICH does not exist so far and diverse clinical trials with hemostatic agent, platelet transfusion, surgical hematoma evacuation, and blood pressure lowering have failed to improve functional outcome after ICH [2].. Thus, it is necessary to develop novel therapeutic strategy based on thorough understanding of neuronal injury mechanism following ICH [2].

Article Title: circRNA Regulates Dopaminergic Synapse, MAPK, and Long-term Depression Pathways in Huntington Disease.
Article Snippet: Huntington disease (HD) is the most common neurogenetic disorder caused by expansion of the CAG repeat in the HTT gene; nevertheless, the molecular bases of the disease are not fully understood.. Non-coding RNAs have demonstrated to be involved in the physiopathology of HD.. However, the role of circRNAs has not been investigated.

Hybridization:

Article Title: Bone Marrow Mesenchymal Stem Cell Exosome Attenuates Inflammasome-Related Pyroptosis via Delivering circ_003564 to Improve the Recovery of Spinal Cord Injury.
Article Snippet: Bone marrow mesenchymal stem cell (BMSC) is previously reported to present a certain effect on treating spinal cord injury (SCI), while the underlying mechanism is largely uncovered.. Therefore, the current study aimed to investigate the involvement of exosome-delivered circRNA profile in the BMSC’s effect on pyroptosis for SCI treatment.. H2O2 treated rat primary neurons were cultured with normal medium, BMSC, BMSC plus GW4869, and BMSC-derived exosome, respectively, then inflammasome-related pyroptosis markers, and circRNA profiles were detected.

Article Title: Potential role of circular RNA in cyclosporin A-induced cardiotoxicity in rats.
Article Snippet: Then, the enriched circRNAs were amplified and transcribed into fluorescent cRNA using a random priming method (Arraystar Super RNA Labeling Kit; Arraystar). .. The labeled cRNAs were hybridized onto the Arraystar Rat circRNA Array (8x15K, Arraystar) and incubated for 17 h at 65 C in an Aligent Hybridization Oven. .. After the slides were washed, the arrays were scanned by an Agilent G2505C scanner.

Microarray:

Expressing:

Article Title: CircMAP3K5 promotes cardiomyocyte apoptosis in diabetic cardiomyopathy by regulating miR-22-3p/DAPK2 Axis.
Article Snippet: .. To investigate the circRNA expression profile of DCM, we have applied the Arraystar Rat circRNA Array analysis of specimen from three DCM samples and three normal controls. .. A total of 14139 circRNAs were detected by Arraystar Rat circRNA Microarray.

Incubation:

Quantitative Proteomics:

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Differential expression of circRNA by A, scatter plot, B, volcanic map, and C, cluster analysis in normal group and diabetic cardiomyopathy (DCM) group. circRNA, circular RNA.

Journal: Journal of Diabetes

Article Title: CircMAP3K5 promotes cardiomyocyte apoptosis in diabetic cardiomyopathy by regulating miR ‐22‐3p/ DAPK2 Axis

doi: 10.1111/1753-0407.13471

Figure Lengend Snippet: Differential expression of circRNA by A, scatter plot, B, volcanic map, and C, cluster analysis in normal group and diabetic cardiomyopathy (DCM) group. circRNA, circular RNA.

Article Snippet: To investigate the circRNA expression profile of DCM, we have applied the Arraystar Rat circRNA Array analysis of specimen from three DCM samples and three normal controls.

Techniques: Expressing

Depletion of circRNA Mitogen‐activated protein kinase kinase kinase 5 (circMAP3K5) attenuates cardiomyocyte apoptosis in a diabetic cardiomyopathy (DCM) cell model. A, quantitative real‐time polymerase chain reaction results show expression levels of circMAP3K5 in samples of DCM ( n = 3) and control ( n = 3) cells. B, Western blots showing expression levels of Bcl‐2, Bax, and cleaved caspase‐3 proteins in the samples. C, Flow cytometry results depict the rate of apoptosis. Statistically significant difference: * p < .05 ( n = 3). circRNA, circular RNA; HG, high glucose.

Journal: Journal of Diabetes

Article Title: CircMAP3K5 promotes cardiomyocyte apoptosis in diabetic cardiomyopathy by regulating miR ‐22‐3p/ DAPK2 Axis

doi: 10.1111/1753-0407.13471

Figure Lengend Snippet: Depletion of circRNA Mitogen‐activated protein kinase kinase kinase 5 (circMAP3K5) attenuates cardiomyocyte apoptosis in a diabetic cardiomyopathy (DCM) cell model. A, quantitative real‐time polymerase chain reaction results show expression levels of circMAP3K5 in samples of DCM ( n = 3) and control ( n = 3) cells. B, Western blots showing expression levels of Bcl‐2, Bax, and cleaved caspase‐3 proteins in the samples. C, Flow cytometry results depict the rate of apoptosis. Statistically significant difference: * p < .05 ( n = 3). circRNA, circular RNA; HG, high glucose.

Article Snippet: To investigate the circRNA expression profile of DCM, we have applied the Arraystar Rat circRNA Array analysis of specimen from three DCM samples and three normal controls.

Techniques: Real-time Polymerase Chain Reaction, Expressing, Western Blot, Flow Cytometry

CircRNA mitogen‐activated protein kinase kinase kinase 5 (circMAP3K5) acts as a sponge for miR‐22‐3p in cardiomyocytes. A, Predicted potential interactions between circMAP3K5 and miR‐22‐3p according to Miranda software. B, quantitative real‐time polymerase chain reaction (qRT‐PCR) results showing expression level of miR‐22‐3p in H9c2 cells. C, Luciferase activity in wild‐type circMAP3K5 (circMAP3K5 WT) and a mutant with a miR‐22‐3p binding site (circMAP3K5 MUT) in 293 T cells transfected with a control mimic or a miR‐22‐3p mimic. D, qRT‐PCR results depicting levels of miR‐22‐3p expression in H9c2 cells transfected with either si‐MAP3K5 NC or si‐MAP3K5. E, qRT‐PCR results showing levels of miR‐22‐3p expression in H9c2 cells transfected with either pLc‐MAP3K5 vector or pLc‐MAP3K5. F, Western blots showing expression levels of the apoptosis regulatory protein. Statistically significant difference: * p < .05 ( n = 3). HG, high glucose; miRNA, microRNA.

Journal: Journal of Diabetes

Article Title: CircMAP3K5 promotes cardiomyocyte apoptosis in diabetic cardiomyopathy by regulating miR ‐22‐3p/ DAPK2 Axis

doi: 10.1111/1753-0407.13471

Figure Lengend Snippet: CircRNA mitogen‐activated protein kinase kinase kinase 5 (circMAP3K5) acts as a sponge for miR‐22‐3p in cardiomyocytes. A, Predicted potential interactions between circMAP3K5 and miR‐22‐3p according to Miranda software. B, quantitative real‐time polymerase chain reaction (qRT‐PCR) results showing expression level of miR‐22‐3p in H9c2 cells. C, Luciferase activity in wild‐type circMAP3K5 (circMAP3K5 WT) and a mutant with a miR‐22‐3p binding site (circMAP3K5 MUT) in 293 T cells transfected with a control mimic or a miR‐22‐3p mimic. D, qRT‐PCR results depicting levels of miR‐22‐3p expression in H9c2 cells transfected with either si‐MAP3K5 NC or si‐MAP3K5. E, qRT‐PCR results showing levels of miR‐22‐3p expression in H9c2 cells transfected with either pLc‐MAP3K5 vector or pLc‐MAP3K5. F, Western blots showing expression levels of the apoptosis regulatory protein. Statistically significant difference: * p < .05 ( n = 3). HG, high glucose; miRNA, microRNA.

Article Snippet: To investigate the circRNA expression profile of DCM, we have applied the Arraystar Rat circRNA Array analysis of specimen from three DCM samples and three normal controls.

Techniques: Software, Real-time Polymerase Chain Reaction, Quantitative RT-PCR, Expressing, Luciferase, Activity Assay, Mutagenesis, Binding Assay, Transfection, Plasmid Preparation, Western Blot

CircRNA mitogen‐activated protein kinase kinase kinase 5 (circMAP3K5) promotes HG‐induced cardiomyocyte apoptosis by regulating the miR‐22‐3p/DAPK2 axis. A, Western blots showing expression levels of DAPK2 protein in cardiac myocytes transfected with either si‐circMAP3K5 or pLcMAP3K5, and exposed to high glucose concentrations (25 mmol/L) for 48 h. B, Western blots showing expression levels of apoptosis‐related proteins in cardiomyocytes transfected with si‐circMAP3K5, miR‐22‐3p inhibitor, and/or pcDNA‐DAPK2, under high glucose concentrations (25 mmol/L). C, Proposed mechanism of circMAP3K5 mediated DCM pathological process: Increases circMAP3K5 levels in the diabetic cardiomyopathy (DCM) heart leading to its interaction with miR‐22, which affects cardiomyocytes apoptosis through upregulation of DAPK2. DAPK2, death‐associated protein kinase 2; HG, high glucose.

Journal: Journal of Diabetes

Article Title: CircMAP3K5 promotes cardiomyocyte apoptosis in diabetic cardiomyopathy by regulating miR ‐22‐3p/ DAPK2 Axis

doi: 10.1111/1753-0407.13471

Figure Lengend Snippet: CircRNA mitogen‐activated protein kinase kinase kinase 5 (circMAP3K5) promotes HG‐induced cardiomyocyte apoptosis by regulating the miR‐22‐3p/DAPK2 axis. A, Western blots showing expression levels of DAPK2 protein in cardiac myocytes transfected with either si‐circMAP3K5 or pLcMAP3K5, and exposed to high glucose concentrations (25 mmol/L) for 48 h. B, Western blots showing expression levels of apoptosis‐related proteins in cardiomyocytes transfected with si‐circMAP3K5, miR‐22‐3p inhibitor, and/or pcDNA‐DAPK2, under high glucose concentrations (25 mmol/L). C, Proposed mechanism of circMAP3K5 mediated DCM pathological process: Increases circMAP3K5 levels in the diabetic cardiomyopathy (DCM) heart leading to its interaction with miR‐22, which affects cardiomyocytes apoptosis through upregulation of DAPK2. DAPK2, death‐associated protein kinase 2; HG, high glucose.

Article Snippet: To investigate the circRNA expression profile of DCM, we have applied the Arraystar Rat circRNA Array analysis of specimen from three DCM samples and three normal controls.

Techniques: Western Blot, Expressing, Transfection

Journal: Journal of Diabetes

Article Title: CircMAP3K5 promotes cardiomyocyte apoptosis in diabetic cardiomyopathy by regulating miR ‐22‐3p/ DAPK2 Axis

doi: 10.1111/1753-0407.13471

Article Snippet: These circRNAs were amplified and transcribed into fluorescent cRNA using a random primer method (Arraystar Super RNA Labeling Kit; Arraystar). labeled cRNAs that were hybridized to Arraystar Rat circRNA Array V2.0 (8 × 15 K) with a total of 14 145 circRNA probes on the chip.

Techniques: Expressing

Journal: Journal of Diabetes

Article Title: CircMAP3K5 promotes cardiomyocyte apoptosis in diabetic cardiomyopathy by regulating miR ‐22‐3p/ DAPK2 Axis

doi: 10.1111/1753-0407.13471

Techniques: Real-time Polymerase Chain Reaction, Expressing, Western Blot, Flow Cytometry

Journal: Journal of Diabetes

Article Title: CircMAP3K5 promotes cardiomyocyte apoptosis in diabetic cardiomyopathy by regulating miR ‐22‐3p/ DAPK2 Axis

doi: 10.1111/1753-0407.13471

Techniques: Software, Real-time Polymerase Chain Reaction, Quantitative RT-PCR, Expressing, Luciferase, Activity Assay, Mutagenesis, Binding Assay, Transfection, Plasmid Preparation, Western Blot

Journal: Journal of Diabetes

Article Title: CircMAP3K5 promotes cardiomyocyte apoptosis in diabetic cardiomyopathy by regulating miR ‐22‐3p/ DAPK2 Axis

doi: 10.1111/1753-0407.13471

Techniques: Western Blot, Expressing, Transfection

The animal model and the overall features of m 6 A-circRNA in morphine-tolerant rats. (A) Morphine-induced antinociception was assessed through the tail-flick test, and tail-flick latency was converted to %MPE ( n = 4), *** P < 0.001 compared with the NS group using Two-way repeated measures of ANOVA followed by Bonferroni’s post-hoc test. (B) Expression of RNA demethylase and methyltransferase determined by qRT-PCR. ( n = 4) ** P < 0.01, *** P < 0.001 compared with the NS group using a two-tailed unpaired Student’s t -test. (C) Expression of RNA methylase determined by qRT-PCR ( n = 4). * P < 0.05 compared with the NS group using a two-tailed unpaired Student’s t -test. (D) Hierarchical clustering shows global m 6 A-methylated circRNAs between the MT group and NS group ( n = 4).

Journal: Frontiers in Neuroscience

Article Title: Identification and characterization of N6-methyladenosine circular RNAs in the spinal cord of morphine-tolerant rats

doi: 10.3389/fnins.2022.967768

Figure Lengend Snippet: The animal model and the overall features of m 6 A-circRNA in morphine-tolerant rats. (A) Morphine-induced antinociception was assessed through the tail-flick test, and tail-flick latency was converted to %MPE ( n = 4), *** P < 0.001 compared with the NS group using Two-way repeated measures of ANOVA followed by Bonferroni’s post-hoc test. (B) Expression of RNA demethylase and methyltransferase determined by qRT-PCR. ( n = 4) ** P < 0.01, *** P < 0.001 compared with the NS group using a two-tailed unpaired Student’s t -test. (C) Expression of RNA methylase determined by qRT-PCR ( n = 4). * P < 0.05 compared with the NS group using a two-tailed unpaired Student’s t -test. (D) Hierarchical clustering shows global m 6 A-methylated circRNAs between the MT group and NS group ( n = 4).

Article Snippet: The labeled cRNAs were combined and hybridized to Arraystar Rat CircRNA Epitranscriptomic Arrays (8x15K, Arraystar) at 65°C for 17 h in an Agilent Hybridization Oven.

Techniques: Animal Model, Tail Flick Test, Expressing, Quantitative RT-PCR, Two Tailed Test, Methylation

The detailed information of significantly top 10 hypermethylated m 6 A-circRNA and top 10 hypomethylated m 6 A-circRNAs.

Journal: Frontiers in Neuroscience

Article Title: Identification and characterization of N6-methyladenosine circular RNAs in the spinal cord of morphine-tolerant rats

doi: 10.3389/fnins.2022.967768

Figure Lengend Snippet: The detailed information of significantly top 10 hypermethylated m 6 A-circRNA and top 10 hypomethylated m 6 A-circRNAs.

Article Snippet: The labeled cRNAs were combined and hybridized to Arraystar Rat CircRNA Epitranscriptomic Arrays (8x15K, Arraystar) at 65°C for 17 h in an Agilent Hybridization Oven.

Techniques:

Joint analysis of m 6 A methylation and expression patterns of circRNAs in morphine tolerance. (A) The nine-quadrant graph shows the relationship between m 6 A quantity and the expression of circRNAs. (B) Cumulative distribution of circRNAs expression between MT group and NS group for m 6 A-circRNAs (blue) and non-m 6 A- circRNAs (red). (C) The upset diagram shows the m 6 A quantity, expression, and m 6 A level of circRNAs and presents 14 different methylation and expression patterns of circRNAs. (D) The dot plot shows the distribution of 96 circRNA with both dysregulated m 6 A quantity and m 6 A level. The orange dots (hyper-up) show 27 circRNAs were hypermethylated and up-expressed, 2 navy blue dots (hypo-down) represent circRNAs were hypomethylated and down-expressed; 1 black dot (hypo-up) represent circRNA were hypomethylated and up-expressed; the light blue dots (hypo-no) represent 66 circRNAs with decreased m6A modification quantity but unchanged expression levels.

Journal: Frontiers in Neuroscience

Article Title: Identification and characterization of N6-methyladenosine circular RNAs in the spinal cord of morphine-tolerant rats

doi: 10.3389/fnins.2022.967768

Figure Lengend Snippet: Joint analysis of m 6 A methylation and expression patterns of circRNAs in morphine tolerance. (A) The nine-quadrant graph shows the relationship between m 6 A quantity and the expression of circRNAs. (B) Cumulative distribution of circRNAs expression between MT group and NS group for m 6 A-circRNAs (blue) and non-m 6 A- circRNAs (red). (C) The upset diagram shows the m 6 A quantity, expression, and m 6 A level of circRNAs and presents 14 different methylation and expression patterns of circRNAs. (D) The dot plot shows the distribution of 96 circRNA with both dysregulated m 6 A quantity and m 6 A level. The orange dots (hyper-up) show 27 circRNAs were hypermethylated and up-expressed, 2 navy blue dots (hypo-down) represent circRNAs were hypomethylated and down-expressed; 1 black dot (hypo-up) represent circRNA were hypomethylated and up-expressed; the light blue dots (hypo-no) represent 66 circRNAs with decreased m6A modification quantity but unchanged expression levels.

Article Snippet: The labeled cRNAs were combined and hybridized to Arraystar Rat CircRNA Epitranscriptomic Arrays (8x15K, Arraystar) at 65°C for 17 h in an Agilent Hybridization Oven.

Techniques: Methylation, Expressing, Modification

Construction of circRNA–miRNA networks in morphine tolerance using circRNAs with differences in both m 6 A quantity and m 6 A modification level.

Journal: Frontiers in Neuroscience

Article Title: Identification and characterization of N6-methyladenosine circular RNAs in the spinal cord of morphine-tolerant rats

doi: 10.3389/fnins.2022.967768

Figure Lengend Snippet: Construction of circRNA–miRNA networks in morphine tolerance using circRNAs with differences in both m 6 A quantity and m 6 A modification level.

Article Snippet: The labeled cRNAs were combined and hybridized to Arraystar Rat CircRNA Epitranscriptomic Arrays (8x15K, Arraystar) at 65°C for 17 h in an Agilent Hybridization Oven.

Techniques: Modification

M 6 A levels of the four circRNAs involved in the circRNA–miRNA networks were verified by MeRIP-qPCR. Relative m 6 A methylation level was calculated as the percentage of modified transcripts in all transcripts. P -values were calculated using Student’s t -test ( n = 4). ** P < 0.01, *** P < 0.001 compared with the NS group using a two-tailed unpaired Student’s t -test.

Journal: Frontiers in Neuroscience

Article Title: Identification and characterization of N6-methyladenosine circular RNAs in the spinal cord of morphine-tolerant rats

doi: 10.3389/fnins.2022.967768

Figure Lengend Snippet: M 6 A levels of the four circRNAs involved in the circRNA–miRNA networks were verified by MeRIP-qPCR. Relative m 6 A methylation level was calculated as the percentage of modified transcripts in all transcripts. P -values were calculated using Student’s t -test ( n = 4). ** P < 0.01, *** P < 0.001 compared with the NS group using a two-tailed unpaired Student’s t -test.

Article Snippet: The labeled cRNAs were combined and hybridized to Arraystar Rat CircRNA Epitranscriptomic Arrays (8x15K, Arraystar) at 65°C for 17 h in an Agilent Hybridization Oven.

Techniques: Methylation, Modification, Two Tailed Test

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